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. 2016 Nov 24;7(11):e2477. doi: 10.1038/cddis.2016.335

Figure 4.

Figure 4

Mtdh facilitates MPC5 apoptosis through the activation of p38 MAPK pathway. (a) p-p38 MAPK expression in the samples obtained from the glomeruli of DN and control rat. (b) Quantification of the results presented in a (n=6). (c) p-p38 MAPK expression in HG-induced MPC5 cells for 12, 24, and 48 h. (d) Quantification of the results presented in c (n=3). (e) Apoptosis of MPC5 cells treated with NG, HG, and HG, and p38 MAPK inhibitor (p38I) for 48 h. (f) Quantification of the results presented in e (n=3). (g) p-p38, Bax, and cleaved caspase 3 expression levels in MPC5 cells treated with NG, HG, and HG, and p38I for 48 h. (h–j) Quantification of p-p38 MAPK (h), Bax (i), and cleaved caspase 3 (j) expressions (n=3). (k) p-p38 MAPK expression levels in NC or si-Mtdh-treated MPC5 incubated with HG for 48 h (n=3). (l) p-p38 MAPK expression levels in control vector or Mtdh overexpression vector-treated cells (n=3). Bars represent mean±S.E.M. *P<0.05, **P<0.01, and ***P<0.001