Figure 3.

Two sites (−126, −172) are necessary for ligase IV promoter activation in retinal neural cells. (a) Two mutations were introduced into the ATF sites in the −601/+164 constructs. (b) Primary retinal neurocytes were cultured in serum-free medium, with 1.0 mM lithium, and transfected with a ligase IV promoter reporter containing the WT or Mut ATF sites, shown in (a). The levels of luciferase activity were normalized to Renilla luciferase (WT, 2.47±0.56; Mt1, 0.286±0.11; Mt2, 0.208±0.072; Mt3, 0.106±0.029). n=3 for each group, **P<0.001. All error bars represent SEM