Figure 1.

Overexpression of NCOA3 enhances breast cancer resistance to taxol. (a) RT-PCR analysis of relative NCOA3 expression in 22 breast cancer tissues from taxol-sensitive patients and 33 breast cancer tissues from taxol-resistant patients. (b and c) Western blot and RT-PCR were performed to detect the protein and mRNA expression of NCOA3 in taxol-resistant MCF-7 cells (MCF-7/Tax1 and MCF-7/Tax2) and taxol-resistant 231 cells (231/Tax1 and 231/Tax2). Data were from three independent experiments. Actin was used as a loading control. Data represent mean±S.D. ***P<0.001. (d and e) (d) MCF-7/Tax1 (A) and 231/Tax1 (B) cells were transfected with NC and NCOA3 siRNA. (e) MCF-7 (A) and 231 (B) cells were transfected with control (CTR) and NCOA3 vectors. After 8 h, cells were treated with indicated dose of Taxol (Tax) for additional 48 h. Cell apoptosis was assessed by Annexin-V-FITC/PI staining assay by flow cytometry. Columns, means of three determinations; bars, S.D.; **P<0.01; ***P<0.001, compared with control treated cells. (f) MCF-7/Tax1 (A) and 231/Tax1 (B) cells were transfected with NC and NCOA3 siRNA for 48 h. (g) MCF-7 (A) and 231 (B) cells were transfected with CTR and NCOA3 vectors for 48 h. Western blot was performed to detect the indicated protein expression. Data were from three independent experiments