Figure 3.

Both miR-17 and miR-20b enhances taxol-induced apoptosis in breast cancer cells. (a and b) (a) 231/Tax1 and MCF-7/Tax1 were transfected with miR-17 mimics (a) or miR-20b mimics (b) and NC for 48 h, respectively. (B) MCF-7 and 231 cells were transfected with miR-17 inhibitors (a) or miR-20b inhibitors (b) and NC for 48 h, respectively. RT-PCR was performed to detect the expression of miR-17 or miR-20b. (c and d) MCF-7/Tax1 (A) and 231/Tax1 (B) were transfected with miR-17 or miR-20b mimics. (e and f) MCF-7 (A) and 231 (B) were transfected with miR-17 or miR-20b inhibitors. After 8 h, cells were treated with indicated dose of taxol (Tax) for additional 48 h. MTT assay was performed to examine cell viability (c and e). Cell apoptosis was assessed by Annexin-V-FITC/PI staining assay by flow cytometry (d and f). Columns, means of three determinations; bars, S.D. *P<0.05; **P<0.01; ***P<0.001, compared with NC-treated cells