Figure 2.

Regulation of NF-κB-dependent gene expression by CTSB and CTSS in LX2 cells. (a) Immunoblot of nuclear extracts, or (b) p65-NF-κB confocal microscopy of LX2 cells exposed to CA-074 (25 μM) or Z-FL (10 μM) overnight, and challenged for 10 min TNF (50 ng/ml), or after 30 min LPS (50 ng/ml). (c) Time course of p65-NF-κB in nuclear extracts of LX2 cells exposed to TNF with/without preincubation with CA-074 (25 μM, overnight). (d) mRNA expression of MCP1 and IL6 in LX2 cells after overnight preincubation with CA-074 or Z-FL followed by exposure to TNF or LPS for 6 h. (e) MCP1 mRNA expression in LX2 cells preincubated overnight with Ten6 (10 μM) or RSV (100 μM) followed by 6 h LPS. (f) MCP1 and IL6 mRNA expression in LX2 cells infected with Control or SIRT1 shRNA, preincubated with CA-074 (25 μM) or Z-FL (10 μM) followed by exposure to 6 h TNF. Results are given as mean±S.D., n=4; *P<0.05 versus control or shControl, and ^¶P<0.05 versus LPS- or TNF-treated cells