Figure 7.

Effect of CTSB/S inhibition on liver inflammation in mice with hepatic fibrosis. (a) After inducing liver fibrosis in mice by CCl₄ injection (twice weekly, 0.5 μl/gr) and after H&E and Sirius Red staining displaying collagen accumulation, mice received CTSB/S inhibitors (10 mg/kg) followed 1 h later by LPS i.p. injection (1.0 mg/kg, 6 h). Then, (b) MCP1 and IL6 mRNA expression was determined in liver tissue, and (c) hepatic SIRT1 activity was analyzed. (a) Results are given as a mean±S.E.M., n=6, *P<0.001 versus Control, ^¶P<0.001 versus LPS, and (b) **P<0.05 versus Ctrl with CCl₄