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. 2016 Nov 10;7(11):e2458. doi: 10.1038/cddis.2016.323

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Silencing of Pref-1 increased adipocyte differentiation in RNase-L knockdown 3T3-L1 cells. (a) The silencing efficiencies of Pref-1 in siPref-1 #1 and #2 transfection compared to siNC transfection in RNase-L gene silencing stable clones of 3T3-L1 pre-adipocytes. Pref-1 expression was measured by qPCR. (b) After induction to differentiation, the lipid accumulation of the siPref-1 #1 and #2 transfected cells and siNC control cells were examined by Oil red O staining (upper) and bright field microscopy imaging (lower). Scale bar represents 50 μm. (c) The lipid content of 3T3-L1 in the experiments in (b) were quantified. The data represented in mean±S.E.M. were from three independent experiments. *P<0.05