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. 2016 Nov 24;7(11):e2480. doi: 10.1038/cddis.2016.225

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Positive correlation between c-jun and FOXK1 expression in GC. (A) Double staining of FOXK1 and c-jun in MKN28 cells in an indirect immunofluorescence assay; the nuclei were counterstained using Hoechst 33258. (B) FOXK1 and c-jun expression levels were detected in GC cell lines and in the immortalized normal gastric epithelial cell line GES-1 using western blot analysis. (C) C-jun (a and c) and FOXK1 (b and d) expression levels in normal or cancerous gastric tissue specimens were detected using immunohistochemical (IHC) assays. Normal mouse IgG was used as the isotype control for the first antibody (a and b). (D) The average scores of the two proteins in normal and cancerous GC tissues. P<0.001 between normal and cancer tissues. (E) FOXK1 and c-jun-positive staining was quantified, and the correlation between these proteins was analyzed using the Spearman's correlation method. P<0.001. (F) Kaplan–Meier overall survival analysis of GC patients. Survival analysis was performed according to the expression status of FOXK1 (a) and c-jun (b), as well as the combined expression status of FOXK1 and c-jun (c). (G) Representative IHC images for tissues are shown. Scale bars represent 20 μm in (A) and 100 μm in (C and G)