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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Nat Struct Mol Biol. 2011 Dec 11;19(1):62–71. doi: 10.1038/nsmb.2169

Figure 6.

Figure 6

Role of the NZF domains in cleaving longer Ub chains. Mammalian TRABID variants were incubated with polyUb substrates for indicated times and visualized by silver staining. (a) Activity of TRABID variants against Lys29, Lys33 and Lys63-linked diUb at indicated time point. FL C443S, catalytic mutant; FL NZFmut, FL with mutations in all three NZF domains; FL ∆Ank, FL lacking AnkUBD; AnkOTU, crystallized fragment; OTU, OTU domain. (b) Time course analysis of mammalian TRABID FL, FL NZFmut and AnkOTU activity towards Lys63-linked hexaUb. (c) Model for the role of the AnkUBD as an S1' Ub binding site in TRABID. (d) Model for the additional contribution of the NZF domains in cleaving longer polyUb chains.