Figure 3.

Strategies of cell wall sub-proteomic approaches of the cell wall, combined with the quantitative imaging technique and reverse genetics, using Arabidopsis mesophyll protoplast. Two approaches result in the comprehensive identification of the cell wall proteins and visualization of cell wall dynamics in the same stages of the protoplasts, leading to the prediction of the relationship between cell wall dynamics and the actions of the cell wall proteins. Furthermore, by use of the protoplasts derived from the T-DNA-insertion line, in which a certain gene of interest is disrupted, quantitative imaging analysis of cell wall regeneration would facilitate the identification of cell wall-related genes responsible for cell wall dynamics and help assign the precise biological role to the gene product.