Table 2. qRT-PCR conditions and primer sequences for analysis of gene expression.

Gene	Primers	Amplicon (bp)
ST3GAL1	5'-CAGAGATGGACGGTCACT-3′ 5′-CAACTGTGGTTTCTGACG-3′	197
ST3GAL2	5′-GTGCCTCCGACTGGTTTG-3′ 5′-GAAGCGGTAGGGGTTCTC-3′	191
ST3GAL3	5′-TATGCTTCAGCCTTGATG-3′ 5′-TTGGTGACTGACAAGATGG-3′	164
ST3GAL4	5′-ATGTTGGCTCTGGTCCTG-3′ 5′-AGGAAGATGGGCTGATCC-3′	176
ST3GAL5	5′-CAAAGCAAGATGAGAAGG-3′ 5′-AAACTTGGGACGACATTC-3′	213
ST3GAL6	5′-TATTATGGGGAACGAATG-3′ 5′-AAAAGGGTGAAACTGATG-3′	195
ST6GAL1	5′-CTTGTTTTCCTGCTCAGA-3′ 5′-GCAAACAGAAGAAAGACCA-3′	166
ST6GAL2	5′-ACGCTGCTGATTGACTCTTCT-3′ 5′-CACATACTGGCACTCATCTAA-3′	160
ST6GALNAC1	5′-CTGGTCTTCTTTCTCTTCG-3′ 5′-GTTGAGGGCATTGTTCTCT-3′	192
ST6GALNAC2	5′-CTTTGCCCTGTACTTCTCG-3′ 5′-CAGCACTGGAATGGAGAGA-3′	205
ST6GALNAC3	5′-GGACAACCTGGTACAAAGT-3′ 5′-TATCTCATTTCCCACCTTC-3′	174
ST6GALNAC4	5′-ACCTGCCTGGACCACCACT-3′ 5′-TCGGCACTGTCGATCTCAG-3′	188
ST6GALNAC5	5′-TGGACGGATACCTCGGAGT-3′ 5′-GTCTGGTCAATCTGGGAGC-3′	121
ST6GALNAC6	5′-ACCTACCCCTCAGCAGACG-3′ 5′-CTTGAGGTTGACAGGTCGG-3′	179
ST8SIA1	5′-TACTCTCTCTTCCCACAGG-3′ 5′-GACAAAGGAGGGAGATTGC-3′	149
ST8SIA2	5′-GTGGTCTTCCTCATCTTCG-3′ 5′-GAGGAGCCGTTTATTACAAC-3′	140
ST8SIA3	5′-ATTCTCTCACCCAGGAACTC-3′ 5′-CAATCCGAACACTATTCTTG-3′	141
ST8SIA4	5′-CAAGAACTGAGGAGCACC-3′ 5′-TTTCCAACCTTCTACATTGTG-3′	140
ST8SIA5	5′-CCTTTGCCTTGGTGACCT-3′ 5′-CATGGACAGCACCTTCACT-3′	152
ST8SIA6	5′-CGGCAAGCAGAAGAATATG-3′ 5′-GCTTTCCACCTCGTAACTC-3′	126
GAPDH	5′-CTCCTCCACCTTTGACGCTG-3′ 5′-TCCTCTTGTGCTCTTGCTGG-3′	175

Abbreviation: qRT-PCR, quantitative reverse transcription-PCR