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. 2016 Dec 1;7(12):e2497. doi: 10.1038/cddis.2016.392

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The presence of wild-type p53 sensitizes cells towards avrainvillamide treatment. (a) Immunoblots showing p53, p21 and actin (loading control) expression levels in OCI-AML3 and MV4-11 cells treated with avrainvillamide (AVA; 0, 0.5 and 1.0 μM) for 24 h. (b) Flow cytometry analysis of p53 expression in OCI-AML3 and MV4-11 cells treated with DMSO (CTR) or 0.5 μM AVA for 24 h. Representative results from a single experiment are shown. US, unstained. (c) Flow cytometric median fluorescence intensity (MFI) quantification of p53 (unstained subtracted, n=3) in OCI-AML3 and MV4-11 cells. Results represent mean±S.E.M. (d) Molm-13 cells were transduced with empty vector (CTR) or shp53 then immunoblotted for p53 expression. Actin is shown as a loading control. (e) Flow cytometry analyses of p53 expression levels in non-irradiated and gamma-irradiated Molm-13 cells transduced with empty vector (CTR) or shp53. (f) Proliferation of Molm-13 cells following transduction with empty vector (CTR) or shp53 and treatment with AVA (0.01 (.01), 0.03 (.03), 0.1, 0.3 or 1 μM) for 24 h. Results represent mean±S.E.M. of the values relative to vehicle controls (n=4; *P<0.05). HEK293 cells were transfected with (g) NPM1wt-ECGFP or (h) NPMc+-ECGFP, and treated with DMSO (CTR) or AVA (0.5 μM) for 24 h or LMB (10 nM) for 3 h. Cells were fixed and analyzed by fluorescence microscopy. All transfection experiments were conducted at least three times; representative images are shown. Arrows indicate localization of NPM1wt and NPMc+ (DAPI indicates nuclei; scale bar, 10 μm)