Figure 1.

miR-206 and miR-140 regulate A549 cell growth. (a) Real-time PCR. Relative fold changes of miR-206 (or miR-140) were decreased in lung adenocarcinoma samples (n=10) compared with para-carcinomas (n=10). (b) In situ hybridization detection of miR-206 or miR-140. The brown color in cell indicates the expression of miRNAs. Bar=50 μm. (c) A549 cell growth according to MTT assay. OD was less in miR-206- (P<0.01) or miR-140- treated A549 cells (P<0.01) compared with negative or control mutant-oligo-treated cultures. (d) miRNAs measured with real-time PCR. miR-206 (or miR-140) were higher in miR-206- (or miR-140)-treated cells compared with scrambled-oligo controls. **P<0.01. miR-206 (or miR-140) treatment versus control treatment. (e) FACS analysis of miR-206-induced apoptosis. (f) Cell cycle distribution of A549 cells transfected with miR-206 and miR-140. All experiments were carried out in triplicate. A significant increase in A549 cells in the G1 phase occurred in miR-206- or miR-140-treated cells compared with control cultures. (g) FACS analysis of miR-140-induced apoptosis. Apoptotic cells are shown in the upper left and right, and lower right quadrants of each panel. Apoptotic cells were increased in miR-206- or miR-140-treated cells compared with scrambled- or mutant mimics (Mu-206 or Mu-140) -treated cells after Annexin V-FITC/PI staining. Negative, vehicle-treated cells without oligos. Scrambled (scram), scrambled oligo control RNA. MiR-206 or miR-140, cells treated with miR-206 or miR-140 oligos. Mu-206 or Mu-140, cells treated with mutation sequence of miR-206 or miR-140. ASO-206 or ASO-140, cells treated with antisense RNA specific to miR-206 or miR-140