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. 2016 Dec 22;7(12):e2527. doi: 10.1038/cddis.2016.433

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HY-SDT promotes the lipid efflux through ABCA1 and inhibits lipid uptake by downregulating CD36 and SR-A in macrophage. (a) Western blot analysis of cholesterol efflux transporters ABCA1, ABCG1 and SR-B1 of macrophage at 6 h after different treatments. (b) Western blot analysis of cholesterol efflux transporters of macrophage at indicated time following HY-SDT. (c) Western blot analysis of cholesterol efflux transporters of macrophage pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. (d) RT-PCR analysis of mRNA expression levels of cholesterol efflux transporters in macrophage pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. (e) Cholesterol efflux to HDL (50 μg/well) in macrophage at 6 h following HY-SDT in the presence of ABCA1 siRNA. (f) Fluorescence microscopy analysis of the uptake of DIL-ox-LDL in macrophage in the presence of CD36 siRNA and SR-A siRNA. Scale bar=100 μm. (g) RT-PCR analysis of mRNA expression levels of cholesterol uptake transporters pre-treatment with CC, ATG5 siRNA and TFEB siRNA at 6 h following HY-SDT. *P<0.05, ***P<0.001 versus control, ^#P<0.05 versus SDT. All values are given as mean±S.D. (error bars) of three independent experiments