Figure 5.

TERT inhibition by BIBR increases DNA damage in TERT-positive cells. (a) TERT-positive 4134/Late, 4134/TERT+, BL41 and BL41/B95.8 cells and TERT-negative 4134/TERT- and U2OS cells exposed for 24 h to BIBR or to DMSO as control, were stained with γH2AX to evaluate DNA damage and analyzed by flow cytometry. Panels from one representative experiment are shown. (b) Levels of γH2AX MFI in BIBR- and DMSO-treated cells. Significant differences between values in BIBR-treated versus DMSO-treated cells are shown: *P<0.05, **P<0.01 and ***P<0.001