Figure 6.

Short-term TERT inhibition by BIBR did not affect telomere. TERT-positive 4134/Late, 4134/TERT+, BL41 and BL41/B95.8 cells and TERT-negative 4134/TERT- and U2OS cells exposed for 72 h to BIBR or to DMSO as control were analyzed for telomere length. (a) Telomere length measured by quantitative multiplex PCR assay. TS values are e means and S.D. (bar) of three separate experiments. (b) Telomere lengths analyzed by TRF by the TeloTAGGG telomere length assay. Panel from one representative experiment is shown. (c) TIF analysis. Representative micrographs showing combined telomere FISH/γH2AX immunofluorescence of 4134/Late cells treated with BIBR at 24 h. From the left: DAPI (nuclear marker, blue), telomere probe (red), γH2AX (DNA damage marker, green), combined Telomere/γH2AX and the merged image. Scale bar: 2 μm