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. 2016 Dec 29;7(12):e2562. doi: 10.1038/cddis.2016.425

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effects of FLU and BIBR+FLU treatments on cell cycle profiles and cell viability in LCLs. Cells were treated with FLU (5 μM) and BIBR (30 μM) plus FLU (5 μM) (BIBR+FLU) and analyzed at 48 and 72 h. DMSO was used as control. (a) Cells were labeled with PI and analyzed by flow cytometry. Panels from one representative experiment are shown. Graphs on right: percentages of cells in G1-, S- and G2/M-phase. Values are means and S.D. (bar) of three independent experiments. (b) Cells were labeled with annexin V/PI and analyzed by flow cytometry. Panels from one representative experiment are shown. Graphs on right: percentages of specific cell death. Values are means and S.D. (bar) of three separate experiments. Significant differences between values in BIBR+FLU-treated versus FLU-treated cells are shown: *P<0.05, **P<0.01 and ***P<0.001