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. 2016 Dec 29;7(12):e2571. doi: 10.1038/cddis.2016.349

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mutation at Thr184 abrogates the biological function and transcriptional activity of ATF1. (a) Colony formation assay showed mutation at Thr184 abrogated the cell colony formation activity of ATF1 using CNE1 cells transfected with various plasmids. (b-d) Mutation at Thr184 abrogated the Bcl-2 transcriptional activity of ATF1 indicated by luciferase assay, qRT-PCR assay and western blotting using CNE1 cells transfected with various plasmids for 24 h. (e) Western blotting showed Ser63 and Thr184 affect their phosphorylations mutually using CNE1 cells transfected with various plasmids for 24 h. The remaining band was the endogenous phosphorylate proteins in the NPC cells as indicated in the first lane 'vector'. *P<0.05