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Y. pestis T3SS YopM is an E3 ubiquitin ligase. (a and b) Immunoblotting showing autoubiquitination by GST-YopM in the presence (a) or absence (b) of E1 and E2, ATP, and Ub. (c) Effects of mutations of Cys 68 on YopM–catalyzed polyubiquition chain synthesis. Ubiquitination assays were carried out using GST-YopM and GST-YopM C68A. All cell-based experiments were performed independently two to three times with comparable results
