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. 2016 Dec 1;7(12):e2495. doi: 10.1038/cddis.2016.402

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fate mapping identifies collagen-producing MMT cells in the fibrotic kidney. Groups of eight LysM-Cre/Rosa26-tdTomato transgenic and littermate wild-type mice underwent unilateral ureteric obstruction (UUO) or sham surgery and were killed 7 days later. (a) Three-color confocal microscopy shows very occasional collagen I-producing Tomato⁺F4/80⁺ cells in sham-operated kidneys, whereas the majority of collagen I-producing cells are Tomato⁺F4/80⁺ macrophage lineage cells in the UUO kidney. Examples of collagen-producing Tomato⁺F4/80⁺ cells in the fibrosing kidney are indicated by arrows. (b and c). Three-color flow cytometric analysis and quantitative data demonstrate that the majority (70-80%) of col I⁺ cells in day 3 and 7 UUO kidneys co-express the Tomato reporter, demonstrating that they originate from the macrophage lineage. Data represent results from eight animals. Scale bar, 20 μM