Table 2. Changes in the expression of genes regulating cell cycle progression in ovarian cancer cells exposed to conditioned medium from senescent HPMCs.

	mRNA change (%)
Target	A2780	OVCAR-3	SKOV-3
Cyclin A1	98±5	103±11	193±2*
Cyclin A2	233±11*	161±4*	98±7
Cyclin B1	340±22*	189±12*	156±4*
Cyclin D1	163±8*	89±34	100±2
Cyclin E2	196±8*	238±6*	246±1*
Cyclin G2	104±22	196±8*	199±8*
Cell division cycle 2	92±12	313±5*	99±1
Ki67	211±12*	98±8	246±6*
HGF	99±3	101±2	166±4*

The results derive from experiments performed with HPMC cultures established from four different patients and are expressed as the percentage of values recorded for cancer cells subjected to conditioned medium from young HPMCs (means±S.D.). Cancer cells were used in duplicates. The calculation of relative change in mRNA was standardized to the GAPDH housekeeping gene. The asterisks indicate significant differences as compared with the cancer cells exposed to the conditioned medium from young HPMCs