Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 1;7(12):e2496. doi: 10.1038/cddis.2016.378

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Dnmt3a is upregulated by AP2α during the CI stage. (a) Illustration of the murine Dnmt3a promoter (−1.7 to +0.1 kb) is shown and two reported AP2α binding sites were identified in the proximal region. (b) Correlation coefficient analysis of AP2α and Dnmt3a mRNA expression profiles during the CI stage. The mRNA expressions of AP2α and Dnmt3a at different time points during the CI stage were determined by real-time PCR and normalized to glyceraldehyde-3-phosphate dehydrogenase. (c) Real-time PCR analysis of the expression of Dnmt3a in AP2α knockdown cells (si-1 and si-2) at ci48 h. The results were analyzed as means±S.D. (n=3). Statistical significance is indicated: ***P<0.001. (d) Western blotting analysis of the expression of Dnmt3a in AP2α knockdown cells at ci48 h. Cyclin-dependent kinase 4 served as the loading control. Quantification analysis of the relative protein level of Dnmt3a in AP2α knockdown cells was shown on the right panel. The results were analyzed as means±S.D. (n=3). Statistical significance is indicated: **P<0.01. (e) A luciferase reporter plasmid of the murine Dnmt3a promoter (−1.7 to +0.1 kb) and an HA-AP2α cDNA plasmid were constructed for dual-luciferase assay. Results were expressed as firefly luciferase activity normalized to renilla luciferase activity. Error bars indicate S.D. (n=3). Statistical significance is indicated: *P<0.05, **P<0.01. Gradient expression of HA-AP2α was detected by western blotting (bottom panel)