Figure 1.

Hypermethylation of the core part in SPOP promoter in CRC. (a) Luciferase assays of five SPOP promoter region constructs were carried out. The ratio of Renilla luciferase to Firefly luciferase was calculated for each experiment. The mean value for each test construct was normalized to the activity of the empty vector. (b) Scheme for the location of the CpG islands in the transcription start region in SPOP gene. The CpG sites are indicated by vertical red lines. The regions for MS-PCR and BSP are indicated. TSS, translation start site. (c) Representative results of methylation analysis by MS-PCR and expression analysis by quantitative PCR in CRC tissues (T) and adjacent normal tissues (N). U, unmethylation; M, methylation. (d) correlation analysis of the MSP results. (e) The survival analysis of CRC patients with methylated and unmethylated SPOP promoter. *P<0.05. Data represent the results from three independent experiments