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. 2016 Dec 29;7(12):e2569. doi: 10.1038/cddis.2016.435

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Acceleration effect of SPOP on cell apoptosis and anoikis in vitro. (a) Cell apoptosis was measured with flow cytometry. The cells were stained with Alexa Fluor 647 Annexin V and 7-AAD. Upper (late apoptotic cells) and lower (early apoptotic cells) right quadrants are counted. (b) Cell anoikis was measured with soft agar assay. (c) Cell apoptosis was measured with Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL). *P<0.05. Data represent the results from three independent experiments