Figure 4.

ERBB3 and AKT2 are direct miR-148a-3p targets. (a) The miR-148a-3p- targeting sites in ERBB3 and AKT2 3′-UTRs were mutated. (b) Decreased ERBB3 and AKT2 expression was observed in miR-148a-3p-transfected T24 and UM-UC-3 cells via qRT-PCR and western blot. (c) miR-148a-3p significantly suppressed the luciferase activity of vector that carried the ERBB3 and AKT2 3′-UTRs but not control vector. (d) Statistical analysis indicated that ERBB3 expression was significantly higher in bladder cancer tissues than in adjacent non-tumor tissues. (e) Representative images of IHC staining of TMA. ERBB3 localized to the membrane. (f) miR-148a-3p was significantly negatively correlated with ERBB3 (r=0.149, P=0.017) and DNMT1 (r=0.108, P=0.041). ERBB3 was significantly negatively correlated with DNMT1 (r=0.116, P=0.042). Error bars represent the S.E. obtained from three independent experiments; *P<0.05. Scale bar=100 μm