Figure 5.

ERBB3 knockdown suppresses bladder cancer cell proliferation. (a) Three different and effective siRNAs were used in our studies to avoid off-target phenomena. (b) CCK-8 assay. The relative cell viability of the Si-ERBB3 of T24 and UM-UC-3 cells was lower than that of NC-treated groups (cell viability of 0 nM was regarded as 1.0). (c) Colony formation assay (representative wells are presented). The colony formation rate was lower for Si-ERBB3 (50 nM)-transfected groups compared with NC (50 nM)-transfected groups. (d) Flow cytometric analysis (representative images are presented) of cell cycle distribution. ERBB3 knockdown induced a significant accumulation of cells in the G1-phase and blocked entry into G1-S. (e) Western blot analysis. Si-ERBB3 (50 nM) inhibited the cell cycle and AKT2 signaling-related proteins in T24 and UM-UC-3 cells. Error bars represent the S.E. obtained from three independent experiments; *P<0.05