Figure 8.

miR-148a-3p/ERBB3/AKT2/c-myc established a regulatory circuit in bladder cancer regulation. (a) Transfection of Si-ERBB3 decreased the expression level of its downstream gene AKT2 and c-myc by qRT-PCR. (b) Transfection of Si-AKT2 decreased the phosphorylation level of AKT and expression of c-myc. (c) Overexpression of c-myc was able to, at least partially, rescue the level of c-myc in the presence of Si-AKT2. (d) Both AKT-Inh and c-myc-Inh decreased the expression of c-myc on both mRNA and protein levels. (e) qRT-PCR. c-myc overexpression decreased miR-148a-3p expression. (f) c-myc overexpression in HEK293T cells increased the luciferase activity of the miR-148a-3p promoter. Co-treatment with c-myc-Inh diminished the increase in luciferase activity. (g) c-myc-binding region in the miR-148a-3p promoter region. Error bars represent the S.E. obtained from three independent experiments; *P<0.05