Figure 6.

Cell death was significantly induced by 5-FU treatment with knockdown of MAS genes. (a) Cell death was analysed by FACS using PI and annexin V. Cells were treated 5-FU for 48 h after treatment with 20 nM siRNA of GLS1 for 24 h (Supplementary Figure 4a). GLS1 knockdown, γH2AX and PARP cleavage was determined by immunoblotting. (b) Cell death was analysed by FACS using PI and annexin V. Cells were treated 5-FU for 48 h after treatment with 20 nM siRNA of MDH2 for 24 h (Supplementary Figure 4b). MDH2 knockdown, γH2AX and PARP cleavage was determined by immunoblotting. (c) Cell death was analysed by FACS using PI and annexin V. Cells were treated 5-FU for 48 h after treatment with 20 nM siRNA of GOT2 for 24 h (Supplementary Figure 4c). GOT2 knockdown, γH2AX and PARP cleavage was determined by immunoblotting. P-values were determined using two-tailed Student's t-tests (ns, not significant; *0.01<P<0.05; **0.001<P<0.01; ***P<0.001)