Figure 4.

Inhibition ASPP2 promotes cell migration. (a) The efficiency of RNAi specifically targeting ASPP2 (Si-ASPP2) in comparison with scramble control (Scr) was confirmed by western blotting (left). β-Actin was used as a loading control. The bands were quantified using Image J software as normalized to β-actin (Right). (b) In vitro wound-healing analysis of the cells transfected with either Scr or Si-ASPP2 cells. Representative photographs at different time points of 24 and 48 h after making the wound were presented. (c) The plot represents the quantitative analysis of wound healing area from three independent experiments. Error bars, mean±S.E.M. (d) Migration rate was detected by a transwell assay in Scr and Si-ASPP2 cells. Representative photographs were presented. (e) The bar graph was the quantitative analysis of transwell assay. Error bars, mean±S.E.M. (n=3 independent experiments). *P<0.05, **P<0.01