Figure 6.

MiR-205/ASPP2 axis promotes cell growth both in vitro and in vivo. (a) Cell proliferation was determined in triplicates at 24-h intervals in the cells transfected with Scr or Si-ASPP2 by MTT assays. Error bars, mean±S.E.M. (n=3 independent experiments). *P<0.05. (b) Cell proliferation was determined by a similar MTT assay as described in a in NC, MiR-205 mimics or MiR-205 mimic+ASPP2 transfected cells. Error bars, mean±S.E.M. (n=3 independent experiments). *P<0.05, miR-205 versus NC; ^&P<0.05, miR-205+ASPP2 versus NC; ^#P<0.05, miR-205+ASPP2 versus miR-205 mimics. (c) Tumor growth in nude mice subcutaneously injected into flanks with SiHa/DsRed control and SiHa/DsRed+MiR-205. Representative photographs of the tumors at different time after inoculation with either SiHa/DsRed control or SiHa/DsRed+MiR-205 cells (right). Tumor volume was measure according to materials and methods. Data are presented as means±S.D. (n=5 per group) (left). *P<0.05, **P<0.01. (d) Photographs of the dissected tumors of either SiHa/DsRed control or SiHa/DsRed+MiR-205 cells (upper). Average of tumor weight was presented in the bar graph. Data are presented as means±S.D. (n=5 per group) (bottom). **P<0.01. (e) Real-time analysis of MiR-205 and ASPP2 in the representative tumors. Histograms represent the mean±S.E.M. from three independent assays. **P<0.01. (f) WB analysis of ASPP2, E-cadherin and Vimentin in the representative tumors. β-Actin was used as a loading control