Figure 3.

RanBP9 and TSSC3 function cooperatively to suppress lung metastasis. MTF cells were transfected as indicated and injected into the tail vein of athymic nude mice to establish an in vivo model of lung metastasis. The animals were killed 63 days later. (a) Percentage of mice bearing lung metastases. (b) Numbers of visible lung metastatic nodules on the surface of the lung. (c) Lung metastasis index (ratio of tumor area to the total tumor and lung area). (d) Mean weight of the lungs. All values in (a–d) are mean±s.d. of the 11 or 12 mice per group. * P<0.05, **P<0.01, ***P<0.001. (e) Representative macroscopic images of the lungs. Arrows indicate metastatic lesions. (f) Representative hematoxylin and eosin-stained images of the lungs shown in (e). (g) Kaplan–Meier overall survival curves