Figure 2.

YOYO3 is a potent and non-toxic reporter of late-stage apoptosis suitable for kinetic analysis in high-content long-duration analysis. (a) MEFs were treated with CHX (50 μg/ml) or STS (1 μM), incubated with YOYO3 (300 nM), and scanned every 2 h for red fluorescence for 22 h. (b) MEFs treated and analysed as in (a) but incubated with DRAQ7 (300 nM). (c) Number of events detected from (a) and (b) were directly compared at 10 and 20 h. Bar graphs depict data from single experiment but representative of results from at least three experiments. (d) MEFs were treated with CHX (50 μg/ml) and ABT-737 (1 μM), and co-incubated with YOYO3 (200 nM), scanned every 2 h for 24 h. Data are representative of at least three experiments. (e) MEFs incubated with Annexin V-488 (1 μg/ml) and either YOYO3 (200 nM) or DRAQ7 (600 nM) were directly compared at several time points for relative toxicity. Data shown are from a single experiment but representative of at least three experiments