Table 1. Suggested processing definitions using the IncuCyte ZOOM.

	Parameter	Radius	Adjustment	Edge	Fill	Area	Eccentricity	Mean intensity	Integrated intensity
Annexin V-FITC	Top-Hat	70	3.0	−30	0	0	0	>3	>500
Annexin V-FITC (puncta exclusion)	Top-Hat	25	3.0	−30	0	>100	0	0	0
DRAQ7	Adaptive	−	0.5	−48	5	>40	0	>1.5	0
YOYO3	Top-Hat	25	1.0	−46	0	>100	0	0	0
DEVD-green	Top-Hat	20	1.5	−37	0	>25	<0.9	0	0
Annexin V-594	Top-Hat	10	2	−35	0	>100	0	0	0
CellTrace	Top-Hat	25	0.5	−25	0	>150	0	>3	0