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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Nature. 2016 Oct 26;539(7629):433–436. doi: 10.1038/nature20128

Extended Data Figure 5. Uph+/− Hand2+/− compound heterozygotes recapitulate Uph KO phenotype.

Extended Data Figure 5

a, Uph+/− Hand2+/− double heterozygote embryos developed a single ventricle, pericardial effusion, and were severely growth restricted by E11.5. Scale bars, 1 mm. b, Uph expression is normal in double heterozygotes, whereas Hand2 was reduced by ~90%, relative to wild-type embryos (n = 5 mice for wild type, Hand2 het and Uph het, n = 3 for double het, n = 2 for Hand2 KO, from 1 of 2 independent experiments; mean ± s.e.m.). c, Immunoprecipitation of RNA using either IgG or WDR5 in HL-1 cells followed by qPCR for Uph revealed no binding to WDR5. The WDR5-interacting lncRNA HOTTIP was used as a positive control (n = 3 biological replicates from 1 of 3 independent experiments; mean ± s.e.m.).