Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Dev Dyn. 2016 Dec 29;246(2):100–115. doi: 10.1002/dvdy.24470

Figure 5.

Figure 5

A) Schematic showing the steps in the cell spreading assay. B) 24 hours after explanting epidermis and treating it with DMSO (control). C) 24 hours after explanting epidermis and treating it with the JNK inhibitor. D) Schematic showing the treatment paradigm for the EGTA adhesion assay. E) Control embryo treated with DMSO only. Inset shows E-cadherin labeling confined to edges of cells. F) Representative embryo treated with DMSO followed by EGTA. White arrows point toward regions where the tissue is dissociating. Inset shows internalized E-cadherin. G) Embryo treated with JNK inhibitor alone. Inset shows E-cadherin labeling confined to cell boundaries. H) Embryo treated with JNK inhibitor followed by EGTA. Inset shows E-cadherin largely confined to the cell boundaries. I) The epidermis of a representative embryo showing the cells containing fluorescein labeled Control MO (green) and E-cadherin labeled in red. I′) Corresponding image from I showing only the E-cadherin labeling in red. I″) Corresponding image from I showing only the E-cadherin labeling in black and white. Insets show representative 1–2 cells at 2X magnification. J) The epidermis of a representative embryo showing the cells containing fluorescein labeled JNK1 MO (green) and E-cadherin labeled in red. J′) Corresponding image from J showing only the E-cadherin labeling in red. J″) Corresponding image from J showing only the E-cadherin labeling in black and white. Insets show representative 1–2 cells at 2X magnification. Black arrows indicate groups of cells with higher levels of E-cadherin at the membrane. K–L′) E-cadherin labeling of epidermis from representative embryos (stage 24–26) that were uninjected (K, K′) or injected with CA-JNK (L, L′). Prime letters are the identical pictures in black and white. Insets show a 2 fold magnified image of a cell from the images. White arrows show what appears as puncta of E-cadherin positive bodies within the cytoplasm and arrowheads point to membranes with reduced E-cadherin. Insets show representative 1–2 cells at 2X magnification. M–N′) Clathrin labeling of epidermis from representative embryos (stage 24–26) that were uninjected (M, M′) or injected with CA-JNK (N, N′). Prime letters are the identical pictures in black and white. Insets show representative 1–2 cells at 2X magnification. White arrows indicate non-specific staining where fluorescence is observed on top of the cell.