Skip to main content
. Author manuscript; available in PMC: 2018 Jan 23.
Published in final edited form as: Vaccine. 2016 Dec 22;35(4):552–556. doi: 10.1016/j.vaccine.2016.12.021

Figure 2.

Figure 2

Pig antibody in vitro neutralization activity against STa toxin, measured by preventing STa toxin from stimulation of cGMP in T-84 cells with an intracellular cyclic GMP EIA kit. The serum or colostrum samples (30 µl) of the immunized dams (□) or the control (■) dams were incubated with STa toxin (2 ng) and then added to T-84 cells. Intracellular cGMP levels (pmole/ml) in the incubated cells were measured based on manufacturer’s protocol (Enzo Life). T-84 cells incubated with STa toxin or cell culture medium alone were used to show stimulation of cGMP by STa enterotoxicity or baseline cGMP levels. Boxes and bars are cGMP means and standard deviations.