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. 2004 Nov 9;2(12):e391. doi: 10.1371/journal.pbio.0020391

Figure 2. RNA Editing of KIAA500 Alu Inverted Repeat.

Figure 2

KIAA0500 is a cDNA of 6,577 nt in length cloned from human brain (AB007969) with a predicted open reading frame of 213 amino acids. Four AtoG discrepancies were present within the coding region of which two lead to an amino acid change (Q/R and S/G, respectively).

(A) Structure of the KIAA500 mRNA with location of Alu elements indicated and the predicted RNA secondary structure according to the MFOLD algorithm. Large open box indicates predicted open reading frame. *, editing sites.

(B) Editing analysis of an exonic Alu element in KIAA0500. Editing sites predicted to change amino acids are indicated. Our analysis revealed a significant percentage of editing (%G) at the nucleotide positions 3518 (27% ± 3%), 3522 (20% ± 3%) and 3625 (6% ± 1%) and additional editing sites with less than 5% editing, whereas parallel analysis of human gDNA confirmed the presence of adenosine at these positions. Editing levels in brain (filled column) and lung (open column, where detectable) are shown above each edited nucleotide.