Table 1. Study summary of 27 rare NTM isolates from Saudi Arabia.
| Isolate | Species | 1Type Strain/ 16S rRNA Similarity % | 2Type Strain/ rpoB Similarity % | Age/Gender | Nationality | Specimen type3 | Clinical relevance4 | Treatment5 | Underlying Conditions6 |
|---|---|---|---|---|---|---|---|---|---|
| 1. | M.arupense | NR_043588/99.6 | JN571215/99.0 | 30/F | Saudi | BAL2 | Yes | RIF, EMB, CLR | HL,COPD,HIV |
| 2. | M.cosmeticum | NR_025787/ 99.8 | AY262742/ 96.8 | 39/M | Saudi | sputum1 | No | - | AS,PTB,HIV |
| 3. | M.cosmeticum | NR_025787/ 99.0 | AY262742/ 96.0 | 48/M | Saudi | sputum7 | Yes | - | Lung Transplant |
| 4. | M.cosmeticum | NR_025787/ 99.2 | AY262742/ 96.6 | 59/M | Yemen | skin3 | - | DM | |
| 5. | M.duvalii | NR_026073/99.1 | AY544907/99.1 | 56/F | Saudi | sputum1 | No | - | NHL,RA |
| 6. | M.duvalii | NR_026073/99.7 | AY544907/99.3 | 63/M | Saudi | peritoneum1 | - | CF,AK | HL,CAPD |
| 7. | M.holsaticum | NR_028945/99.4 | AY859705/98.6 | 47/M | Saudi | cecum2 | - | EMB,OF | DM,UC,RA |
| 8. | M.insubricum | NR_125525/99.3 | EU605693/90.0 | 52/M | Eritrea | skin1 | - | - | PTB |
| 9. | M.iranicum | KU861842/99.6 | JQ90669809/98.9 | 51/M | Saudi | BAL2 | Yes | - | PTB |
| 10. | M.kubicae | HM022200/99.3 | 69/F | Saudi | BAL1 | Yes | RA | ||
| 11. | M.kubicae | HM022200/99.7 | 49/M | Saudi | sputum1 | No | - | PTB | |
| 12. | M.kubicae | NR_025000/99.1 | 51/F | Saudi | sputum3 | Yes | - | DM,CVD,RA | |
| 13. | M.kyorinense | NR_041663/98.7 | JQ717032/99.2 | 14/M | Saudi | lymphnode1 | EMB, CLR | HL | |
| 14. | M.monascence | NR_041723/99.5 | HM229793/99. | 68/M | Saudi | sputum6 | Yes | - | PTB,AS |
| 15. | M.monascence | NR_041723/99.3 | KU361327/99.0 | 52/M | Saudi | sputum2 | Yes | - | COPD, BE |
| 16. | M.monascence | NR_041723/99.0 | HM229793/99.0 | 2/F | Saudi | BAL1 | Yes | - | COPD |
| 17. | M.monascence | NR_041723/99.6 | KU361327/99.6 | 52/M | Saudi | Ascitic fluid | - | COPD, IBD | |
| 18. | M.monascence | NR_041723/99.4 | HM229793/98.4 | 63/M | Saudi | Lymphnode | - | BE | |
| 19. | M.novocastrense | NR_029208/99.2 | AY859704/98.9 | 74/M | Saudi | sputum2 | Yes | - | BE,RA |
| 20. | M.perigrinum | NR_114447/99.1 | AY147166/98.7 | 48/M | Sudan | skin1 | - | - | PTB |
| 21. | M.marinum | NR_113366/ 99.2 | CP000854/ 98.2 | 33/M | Sudan | skin1 | RIF,CLR | - | |
| 22. | M.terrae | NR_117886/99.0 | JN571264/98.9 | 68/M | Saudi | sputum1 | No | AK,CF | COPD, DM |
| 23. | M.terrae | NR_115677/99.1 | AY544967/99.0 | 49/M | Nigeria | skin | - | PTB,AS | |
| 24. | M.triplex | NR_117226/99.1 | AY544970/99.0 | 69/M | Saudi | BAL | Yes | - | CVD,AS |
| 25. | M.triplex | NR_117226/99.5 | KF856622/98.7 | 48/F | Saudi | lymphnode | AK, EMB,CLR | HIV | |
| 26. | M.tusciae | NR_117885/99.3 | 64/M | Saudi | sputum3 | Yes | INH, EMB,RIF | BE, HCA | |
| 27. | M.yongonense | KF_224994/99.8 | JF271829/99.1 | 16/M | Saudi | BAL2 | Yes | - | COPD,NHL |
1 16S rRNA gene closest match- Gene bank accession number.
2 rpoB gene closest match- Gene bank accession number.
3 Number shows how many times the species was isolated from the patient.
4 ATS and IDSA NTM pulmonary disease criteria met.
5 Drugs (on use while collecting isolates)- EMB- Ethambutol; CLR- Clarithromycin; AK-Amikacin; RIF-Rifampicin; CF-Ciprofloxacin, OF-Ofloxacin.
6 HL-Hodgkin Lymphoma; DM- Diabetes Mellitus, NHL- Non-Hodgkins Lymphoma; UC-Ulcerative Colitis; RA- Rheumatoid Arthritis; PTB- Previous Tuberculosis; BE- Bronchiectasis; COPD- Chronic Obstructive Pulmonary Disease; IBD- Inflammatory Bowel Disease; HCA- Hepatocellular Carcinoma; AS- Asthma; CAPD- Continuous Ambulatory Peritoneal Dialysis, CVD- Cardio Vascular Diseases, INH-Isoniazid.