Fig 3. Neutrophils display antifungal activities.

(A) Imaging of hyphae and neutrophil behavior within interaction channels (i) demonstrates robust recruitment of neutrophils (Hoechst, open blue arrowheads) to EGFP-expressing fungi (open green arrowheads) and formation of swarms (ii, open blue arrowheads) while production of reactive oxygen species (iii, ROS, open magenta arrowhead) can be visualized by staining with CellROX. (B) Killing of hyphae by neutrophils can be visualised by quenching of cytoplasmic GFP fluorescence following breach of the hyphal cell wall. (C) Neutrophils attach to the hyphae (i) and undergo NETosis (ii). Production of ROS (iii) and formation of new lateral branches (iv) can be observed at sites of neutrophil interaction.