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. 2004 Sep 29;5:73. doi: 10.1186/1471-2164-5-73

Table 2.

Information contained in a comprehensive cross list of soybean unigene clone IDs. Shown are various identifiers and annotations for 27,513 reracked cDNAs used in microarray construction. The full list is provided with arrays and available upon request.

Cross List Identifiers (for each cDNA clone) Example (one of 27,543 cDNAs) Comments
Reracked Clone ID Gm-r1021-12 The individual cDNA clone ID in the 384-well destination plates after reracking or rearraying of the selected clones from the cDNA source library plates.
Reracked Plate ID Gm-r1021 #1 The 384-well reracked plate name in increments of 384 (ie., 1, 385, 769, etc.)
Reracked row_column position A12 Position of the clone in the 384-well reracked plate
Reracked 3' Keck Sequence ID GM210001A21A6 Sequence identifier assigned by the Keck Center for the 3' EST
Reracked 3' Genbank Accession AW348131 Genbank assigned accession number for the 3' EST
Reracked 3' Annotation glutathione S-transferase GST 22 [Glycine max] Top BLASTX hit for the 3' EST, at E10-6 or lower
Source Clone ID Gm-c1004-464 The individual cDNA clone ID in the 384-well source plate.
Source Plate ID Gm-c1004 #385 The 384-well source plate name in increments of 384 (ie., 1, 385, 769, etc.)
Source row_column position D8 Position of the clone in the 384-well source plate
Source WashU Sequence ID sa26h04.y1 Sequence identifier assigned by Washington University, 5' EST
Source 5' Genbank Accession AI442436 Genbank assigned accession number for the 5' EST
Source 5' Annotation glutathione S-transferase GST 22 [Glycine max] Top BLASTX hit for the 5' EST at E10-6 or lower
Source Library Gm-c1004 Name of the cDNA source library
Cultivar/Genotype Williams Specific information on the soybean variety or genotype
Tissue/Developmental Stage Entire roots of 8-day old seedlings Tissue/organ system/stage from which the cDNA library was constructed