Table 2.

Sporozoite numbers in midgut (MG), hemolymph (HL) and salivary glands (SG). Sporozoites were counted at day 14, 17/18, 20 and 22 post infection. The mean and the standard deviation (± SD) of countings from two to three different feeding experiments are shown. Note that not all dissected mosquitoes were infected and hence numbers per infected mosquito are higher.

DOI: http://dx.doi.org/10.7554/eLife.19157.011

Parasite line	No. of MG sporozoites per mosquito	No. of HL sporozoites per mosquito	MG / HL	No. of SG sporozoites per mosquito
wt	18,100 (±10,600)	1,400 (±1,700)	13	7,800 (±5,300)
trp1(-)	42,400 (±12,500)	100 (±100)	424	0
trp1(-)mCh	101,200 (±46,800)	800 (±300)	127	0
sera5(-) fluo	35,700 (±11,900)	0	/	0
sera5(-) non-fluo	50,100 (±12,900)	0	/	25 (±50)
gfp-trp1comp	45,600 (±20,200)	3,500 (±2,900)	13	8,900 (±6,800)
gfp-trp1	9,800 (±10,200)	1,500 (±2,100)	7	1,900 (±2,000)
gfp-trp1∆N	18,900 (±12,700)	1,000 (±700)	19	0
gfp-trp1∆C	52,700 (±9.500)	1,100 (±300)	48	0
trp1-gfp parental	3,400 (±1,600)	n.a.	n.a.	175 (±100)
trp1-gfp clonal	9,300 (±4,500)	2,600 (±1,500)	4	600 (±750)