Table 3.
Infectivity of parasite lines to C57/BL6 mice. Data are shown for the TRP1-knockout lines trp1(-) and trp1(-)mCh as well as for the TRP1 complementations gfp-trp1comp, gfp-trp1∆N and gfp-trp1∆C in comparison to wild-type (wt – P. berghei strain ANKA) and gfp-trp1. MG – midgut; i.v. — intravenous injection into tail vein.
|
Parasite line |
Route of inoculation |
Mice positive #/# |
Prepatency |
|---|---|---|---|
|
wt |
by bite |
4/4 |
3.25 |
|
wt |
500,000 MG Sporozoites (i.v.) |
3/4 |
6.0 |
|
trp1(-) clone 1 |
by bite |
0/4 |
∞ |
|
trp1(-) clone 3 |
by bite |
0/4 |
∞ |
|
trp1(-) clone 3 |
400,000 MG sporozoites (i.v.) |
2/4 |
6.0 |
|
trp1(-)mCh |
by bite |
0/4 |
∞ |
|
trp1(-)mCh |
500,000 MG sporozoites (i.v.) |
4/4 |
6.5 |
|
gfp-trp1comp |
by bite |
4/4 |
3.0 |
|
gfp-trp1 |
by bite |
4/4 |
3.5 |
|
gfp-trp1∆N |
by bite |
0/4 |
∞ |
|
gfp-trp1∆C |
by bite |
0/4 |
∞ |