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. 2016 Nov 18;2(11):e1600844. doi: 10.1126/sciadv.1600844

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Copyright © 2016, The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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Fig. 7 — (A) Immunohistochemical detection of NRG1 (brown arrowheads) in normal and healing (3 dpa) zebrafish hearts. (B) Positive immunofluorescence detection of NRG1 (green) at the ventricular apex of normal zebrafish heart (nzH) and healing zebrafish heart (hzH) but not in the adult mouse heart (mH). (C) Consistent with the in situ NRG1 staining, Western blotting showed that both nzECM and hzECM ECM contain NRG1 protein. (D) Quantification data indicate hzECM and nzECM contain approximately 6.5 and 5 times more NRG1 than normal mECM, respectively. Data represent means ± SD. ***P < 0.001 versus mECM. Scale bars, 50 μm.