Table 1.
Immunogenetics of VelocImmune Mouse Antibodies
| Name | VH | JH | CDR H3 Lengtha |
VH Mut Freqb |
Isotype | VL | JL | CDR L3 Lengtha |
VL Mut Freqb |
|---|---|---|---|---|---|---|---|---|---|
| Antibodies isolated from mouse RE504 | |||||||||
| RE504-46 | 3-30 | 4 | 15 | 4.6% | IgG1 | VK1-9 | 4 | 9 | 3.1% |
| RE504-60 | 3-23 | 2 | 18 | 4.2% | IgG2 | VK3-20 | 5 | 8 | 1.5% |
| RE504-97 | 4-59 | 4 | 14 | 6.3% | IgG2 | VK4-1 | 4 | 9 | 2.2% |
| RE504-117 | 3-33 | 4 | 8 | 3.3% | IgG2 | VK2-24 | 4 | 9 | 0.0% |
| RE504-125 | 4-39 | 6 | 11 | 7.6% | IgG1 | VK1-5 | 5 | 8 | 2.7% |
| Antibodies isolated from mouse RE505 | |||||||||
| RE505-11 | 3-9 | 4 | 9 | 7.3% | IgG1 | Vλ3-01* | 2 | 13 | 4.7% |
| RE505-22 | 3-9 | 4 | 9 | 4.9% | IgG1 | Vλ3-01* | 2 | 13 | 3.1% |
| RE505-58 | 3-9 | 4 | 9 | 5.2% | IgG1 | Vλ3-01* | 2 | 13 | 3.1% |
| RE505-70 | 3-9 | 4 | 9 | 7.3% | IgG1 | Vλ3-01* | 2 | 13 | 3.9% |
| RE505-23 | 3-48 | 4 | 14 | 5.2% | IgG1 | VK3-11 | 2 | 10 | 1.5% |
| RE505-27 | 3-21 | 6 | 17 | 9.0% | IgG1 | VK1-6 | 2 | 9 | 4.2% |
| RE505-33 | 3-23 | 4 | 18 | 5.2% | IgG2 | VK3-20 | 4 | 8 | 1.9% |
a
CDR3 length calculated using IMGT definition of CDR3 after invariant cysteine and before invariant tryptophan
b
Mutation frequency calculated as percentage of nucleotide mutations in V gene segment ending at second invariant cysteine
Underlined names denote antibodies have been inferred to be clonally related