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. Author manuscript; available in PMC: 2018 Feb 20.
Published in final edited form as: Neuroscience. 2016 Dec 21;343:372–383. doi: 10.1016/j.neuroscience.2016.12.020

Fig 3. Axonal regeneration after injury following a single intravitreal injection of Wnt3a.

Fig 3

(A–D) CTB-labeled axons showing increased number of axons past the crush region (*) in Wnt3a injected eyes following axonal injury, compared to saline injected animals. (A–B) Optic nerves at 2 weeks following crush. (C–D) Optic nerves at 4 weeks following crush (inset in D shows non-straight morphology and extension past the crush site of regenerating axons from the bracketed region). (E–F) Quantification of axon regeneration at 2 weeks (E) and 4 weeks (F) post-crush showing progressive growth of axons in the Wnt3a-injected mice. n=6 mice for Wnt3a, n=4 for Saline, *p<0.05. ((E) t-test, 100 μm: t = 2.649, df 8, p = 0.0293; 200 μm: t = 4.396, df 8, p = 0.0023; 300 μm: t = 5.125, df 8, p = 0.0009; 400 μm: t = 2.485, df 8, p = 0.0378; 500 μm: t = 2.296, df = 8, p = 0.0508 (ns); 600 – 800 μm: ns. (F) t-test, 400 μm: t = 3.201, df = 10, p = 0.0095; 500 μm: t = 8.043, df = 8, p<0.0001; 600 μm: t = 7.5434, df = 5, p = 3.53169E-05; 700μm: t = 8.5118, df = 5, p = 1.37372E-05; 800 μm: t = 5.1521, df = 5, p = 0.0002; 900 μm: t = 7.8299, df = 5, p = 2.61393E-05; 1000 μm: t = 5.3913, df = 5, p = 0.00022; 1100 μm: t = 4.5836, df = 5, p = 0.00042; 1200 μm: t = 2.0902, df = 5, ns). Scale bar = 100μm.