Fig 4. Stat3 signaling is upregulated by Wnt3a.
(A) Representative Western blots of total Stat3 (T-Stat3) and phospho-Stat3 (P-Stat3). Retinas from three animals are shown for each group. (B) Quantification of the Western blots demonstrated that Wnt signaling increased Stat3 activation (ratio of phosphorylated Stat3 to total Stat3 expression) two weeks following optic nerve injury by 2.4 fold. n=4 mice per group, *p<0.05 (t-test, t = 3.293, df = 6, p = 0.0166). Total protein amount was normalized to β-actin levels.