Figure 1.

PTX3 accumulates in the extracellular matrix of injured and regenerating skeletal muscles. PTX3 expression was assessed by immunohistochemistry, in sections of WT mouse quadriceps muscles isolated (A) before and (B) 1, (C) 3, (D) 5 and (E) 7 d after injury. Hematoxylin stained nuclei. Scale bars = 20 μm; n = 15 (three mice/time point). Three independent experimental cohorts were studied. (F) CD45⁺ cells were retrieved from WT mouse quadriceps muscles by enzymatic digestion at various times after injury, and the number of F4/80⁺ (black line) and Ly6G⁺ (dashed line) were assessed. Results are expressed as mean ± s.e.m; n ≥ 15 (three–five mice/time point) per three independent experimental cohort.