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. 2016 Nov 23;22:809–820. doi: 10.2119/molmed.2016.00002

Figure 6.

Figure 6.

PTX3 deletion does not per se influence macrophage ability to recognize and phagocytose apoptotic cells. (A) The ability of macrophages propagated from bone marrow precursors of WT or PTX3-/- mice was assessed by flow cytometry using two different phagocytes: apoptotic cell ratios are expressed as percentage of phagocytized cells. Results (mean values ± s.e.m) are representative of three independent experiments performed in triplicate. (B) Representative dot plots of WT and PTX3-/- CD11c+ macrophages (y axis) challenged at 37°C (upper panels) and at 4°C (lower panels) with CMTMR (x axis). Expression of (C) MerTK, (D) Gas6, (E) CD163 and (F) MFG-E8 assessed by RT-PCR in WT and PTX3-/- quadriceps at d 1, 3, 7, 10 and 15 after injury. Results (mean values ± s.e.m) are representative of at least three independent experiments (n ≥ 15, at least three mice for each time point). *: significantly different from WT mice, P < .05 (ANOVA).