. Author manuscript; available in PMC: 2018 Mar 1.

Published in final edited form as: Biochim Biophys Acta. 2016 Dec 6;1862(3):283–290. doi: 10.1016/j.bbalip.2016.12.001

Table 1.

Steady state kinetic analysis of LmBuk at 37°C.

Substrate	k_cat (min⁻¹)	K_M (mM)	k_cat / K_M (mM⁻¹min⁻¹)
Straight-chain
Propionate	155.6 ± 0	174.1 ± 68.9	0.89
Butyrate	50.2 ± 0.4	50.5 ± 5.8	0.99
Pentanoate	71.7 ± 2.2	13.1 ± 3.3	5.5
Hexanoate	1310.9 ± 474.9	1856 ± 676	0.7
Branched-chain
Iso butyrate	23.7 ± 0.4	7.6 ± 1.3	3.1
Isovalerate	25 ± 0.1	57.1 ± 9.6	0.44
2-methylbutyrate	15.9 ± 1.5	36.2 ± 5.2	0.44
2-ethyl butyrate	6.7 ± 1.7	16.5 ± 0.9	0.4
2-methyl pentanoate	18.8 ± 2.4	44.2 ± 8.5	0.4
3-methyl pentanoate	21.2 ± 0.4	27.3 ± 0.8	0.8

Initial velocities were measured at 37 °C in the presence of 10 mM ATP at pH 7.5. The k_cat and K_M were calculated from the least squares fit of the experimental data from the different substrates to the Michaelis-Menten equation. The values indicated are the mean of experiments performed at least in triplicate ± SEM.