Figure 8. Hemolytic activity of the mannose EII subunit mutants.

Hemolytic activity of culture lysates against red blood cells (RBCs) for manLMN mutants in 5448 and MGAS5005 was assayed as described in Experimental Procedures. Data from three biological replicates is shown comparing occurrence of hemolysis (defined as OD₅₄₁ > 0.2) on the X-axis and the relative level of hemolytic activity observed on the Y-axis. Hemolysis at an OD₆₀₀ above 1 was considered normal based on WT 5448 or MGAS5005 (black bars to right of dotted line). Occurrence of hemolysis was noted as early if it occurred before OD₆₀₀ of 1 (red bars left of dotted line).